Strýcová was a strong junior player, winning two Grand Slams in girls' singles: the 2002 Australian Open and then defending that title the following year. She also won three Grand Slam girls' doubles titles between 2001 and 2003. She reached world No. 1 in both singles and doubles on the junior rankings, achieving both in 2002, and was named the ITF Junior World Champion that same year.
Záhlavová-Strýcová defeated Marina Erakovic in the final of the 2011 Bell Challenge to claim her first WTA singles title. In October she won the Linz Open, beating Magdalena Rybarikova 6-4, 6-1. This was her second WTA title.
Strycova has also won 24 WTA doubles tournaments and 5 Fed Cup titles with the Czech Republic.